Generator
3-MBG
Part:BBa_J45400:Experience
Designed by: MIT IGEM 2006 Group: iGEM06_MIT (2006-08-21)
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how you used this part and how it worked out.
Applications of BBa_J45400
Isoamyl alcohol (banana odor precursor) production.
User Reviews
UNIQb77445961346630c-partinfo-00000000-QINU
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BBa_J45400, when used in the banana odor biosynthetic system BBa_J45900, may not be functional since no isoamyl acetate is detected. |
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Characterization
Gas chromatography analysis of banana odor biosynthetic system
The banana odor biosynthetic system (BBa_J45900) includes the isoamyl alcohol generator (BBa_J45400).
No isoamyl acetate production from cellular metabolites by banana biosynthetic system
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.